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Image Search Results
Journal: Journal of Cell Science
Article Title: A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3
doi: 10.1242/jcs.081679
Figure Lengend Snippet: 1m treatment induces differentiation towards definitive endoderm. Shef-3 hESCs were cultured for a total of 7 days, feeder-free on Matrigel, in chemically defined mTeSR1 medium with 2 μM 1m included in the medium for the number of days indicated (0d, hESCs cultured for 7 days without 1m). ( A ) RT-PCR analyses were performed using primers selective for the genes specified. ( B ) Shef-3 hESCs cultured under the conditions indicated for the duration of the experiment in Lumox 24-well trays were analysed by immunofluorescence for expression of brachyury, PDGFRβ, FOXA2, SOX17 and HNF4α. Cells were counterstained with DAPI for nuclear localization and the lower panels show the superimposed images. Scale bars: 50 μm. DMSO controls were cultured for the entire 7-day period.
Article Snippet: Antibodies against the following proteins were used: brachyury (4 μg/ml, goat; AF2085, R&D Systems),
Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Expressing